Introduction ============ Pysiology is a python package developed with the intent of make physiological analysis accessible to researchers worldwide. PySiology is a tool designed with novice users in mind, and it provides a simple way to estimate features from ECG, EMG and EDA signals. Features estimation is done chaining functions, and for each method default value are provided. This allows for a fast estimation of more than 50 features. Furthermore, PySiology provides tutorials that allows not only to learn how to use the package, but also the methodologies behind its modules. Expert users can customize each estimation parameter, add their own and share their results with other users.